the co-culture with vero cells and the development of two cell mouse embryos after vitrification
نویسندگان
چکیده
introduction: the mouse embryos can successfully be vitrified using ethylene glycol as cryoprotectant, however their development differs significantly from the non-vitrified embryos. the ability of their development can be improved when they co-culture with somatic cells. in the present study, the effects of co-culturing with vero cells on the development of vitrified two cell mouse embryos were studied. materials and methods: two cell embryos were flushed from the excised uteri of superovulated mice. morphologically normal embryos were vitrified using 10% ethylene glycol solution (efs10), and thawed rapidly using 0.5 m sucrose solution. the survived embryos were divided to two grops: one group cultured on rpmi and the another one co-cultured with vero cells. control embryos was considered for each experimental groups. results: the survival rate was 83%. the developmental rate of embryos which could reach to morula stage for the exp. group 1 and 2 were 52% and 69% respectively which the difference between the exp. groups was significant (p<0.001). mean while 48% of the exp. group 1 could form blastocysts, accordingly the second group showed 60% and the difference was significant (p<0.001). the difference between the two exp. groups which could reach to hatching stage was not significant. conclusion: the results suggest that the co-culture with vero cells can improve the development of vitrified two cell mouse embryos
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عنوان ژورنال:
cell journalجلد ۱، شماره ۳، صفحات ۱-۷
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